Chemistry is an experimental science, SDS of cas: 2495-37-6, and the best way to enjoy it and learn about it is performing experiments.Introducing a new discovery about 2495-37-6, Name is Benzyl methacrylate, molecular formula is C11H12O2, belongs to quinuclidines compound. In a document, author is Yildiz, Umit Hakan.
Real-time determination of the activity of ATPase by use of a water-soluble polythiophene
This contribution introduces a fluorescence assay for real-time determination of the activity of p97/VCP, a 540-kDa homo-hexameric enzyme, belonging to the AAA-ATPase family. A fluorescent reporter poly 1-(3-((4-methylthiophen-3-yl)oxy)propyl)quinuclidin-1-ium (poly PTQ) is used to monitor the hydrolysis of ATP to ADP by p97/VCP. The proposed assay relies on the different strength of coordination of ATP and ADP to the polymer backbone. We used recovery of fluorescence intensity on addition of p97/VCP to a poly PTQ/ATP solution to determine the enzymatic activity. The kinetic data K (m) and V (max) were 0.30 mmol L-1 ATP and 0.134 nmol ATP min(-1) mu g(-1) enzyme, respectively. The specificity of the assay was investigated by using an unhydrolyzable ATP analogue and sensitivity against p97 mutagenesis was further examined by detection of the activity of wild type and truncated p97/VCP. Our study demonstrates that determination of the real-time activity of p97/VCP is possible, because of the superior sensitivity and very fast optical response of poly PTQ.
Sometimes chemists are able to propose two or more mechanisms that are consistent with the available data. If a proposed mechanism predicts the wrong experimental rate law, however, the mechanism must be incorrect.Welcome to check out more blogs about 2495-37-6, in my other articles. SDS of cas: 2495-37-6.
Reference:
Quinuclidine – Wikipedia,
,Quinuclidine | C7H13N | ChemSpider